Impact and related mechanism of exogenous receptor activity modifying protein 1 on calcitonin gene-related peptide modified bone marrow mesenchymal stem cells on the migration of vascular smooth muscle cells in vitro

• Published in: Zhōnghuá xīnxuèguănbìng zázhì Vol. 43; no. 6; p. 537
• Main Authors: Long, Xianping, Cui, Can, Chen, Panke, Wang, Song, Wang, Dongmei, Xu, Guanxue, Yao, Xiaojian, Shi, Bei
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2015
• Subjects: Animals; Bone Marrow Cells; Calcitonin Gene-Related Peptide; Cell Movement; Coculture Techniques; Hematopoietic Stem Cells; In Vitro Techniques; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; NF-kappa B; Rats; Receptor Activity-Modifying Protein 1; Signal Transduction; Transfection
• ISSN: 0253-3758
• DOI: 10.3760/cma.j.issn.0253-3758.2015.06.015

To investigate the impact of calcitonin gene-related peptide (CGRP) modified bone marrow mesenchymal stem cell (MSC) on the migration of vascular smooth muscle cell (VSMC) and related mechanisms. The MSC and VSMC were isolated from rats and cultured, CGRP was transfected to MSC with the high expression lentivirus vector, VSMC was transfected with high expression lentivirus vector of receptor activity modifying protein 1 (RAMP1) and the silence expression lentivirus vector of RAMP1. Then MSC was co-cultured with VSMC. Experimental groups were as follows: (1) Ang II group (MSC + VSMC + Ang II); (2) MSC(CGRP+) group (MSC(CGRP+) + VSMC + Ang II); (3) MSC(CGRP+) RAMP1(-) group (MSC(CGRP+) + VSMC(RAMP1-) + Ang II); (4) MSC(CGRP+) RAMP1(+) group (MSC(CGRP+) + VSMC(RAMP1+) + Ang II); (5) RAMP1(+) group (MSC + VSMC(RAMP1+) + Ang II). Transwell assay was applied to detect the migration of smooth muscle cells, Western blot was applied to detect the protein expression of cells in various groups. VSMC migration number was