Effects of transient exposure to high glucose on biological behaviors of human dermal microvascular endothelial cells

• Published in: Zhonghua shao shang za zhi Vol. 33; no. 2; p. 77
• Main Authors: Qiao, L, Yang, H Z, Li, X C, Huang, X Q, Yuan, B, Zhou, Z D
• Format: Journal Article
• Language: Chinese
• Published: China 20.02.2017
• Subjects: Apoptosis - drug effects; Biological Products; Cell Movement - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Endothelial Cells; Glucose - pharmacology; Humans; Tissue Inhibitor of Metalloproteinase-3; Cell proliferation; Diabetes mellitus; Microvascular endothelial cells; Cell movement; Neovascularization; Apoptosis; Refractory wound
• ISSN: 1009-2587
• DOI: 10.3760/cma.j.issn.1009-2587.2017.02.004

To observe the effects of transient exposure to high glucose on biological behaviors of human dermal microvascular endothelial cells cultured in vitro. The dividing method and treatment of cells for the detection of all indexes in this study were as follows. Human dermal microvascular endothelial cells of the 4th passage were divided into 3 groups according to the random number table, with 12 wells in each group. Cells in control group (C) were cultured with complete culture solution containing 5 mmol/L D-glucose for 7 d. Cells in transient high glucose group (THG) were cultured with complete culture solution containing 30 mmol/L D-glucose for 2 d and complete culture solution containing 5 mmol/L D-glucose for 5 d. Cells in prolonged high glucose group (PHG) were cultured with complete culture solution containing 30 mmol/L D-glucose for 7 d. (1) The cell morphology in groups C and PHG on culture day 7 and that in group THG on culture day 2 and 7 was observed by inverted optical microscope. (2) On culture day