The Role of DNA Double-strain Damage Repairing Mechanisms in Diabetic Atheroscolersis
To identify the role of DNA double-strain damage repairing pathway in the development of diabetics atherosclerosis. Wistar male rats were randomly divided into three groups: control group (group A), balloon injury group (group B) and diabetes + balloon injury group (group C). Streptozotocin (STZ) wa...
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Published in | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 48; no. 2; p. 191 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.03.2017
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Subjects | |
Online Access | Get more information |
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Summary: | To identify the role of DNA double-strain damage repairing pathway in the development of diabetics atherosclerosis.
Wistar male rats were randomly divided into three groups: control group (group A), balloon injury group (group B) and diabetes + balloon injury group (group C). Streptozotocin (STZ) was injected into rat abdomen to induce diabetes. After stabilizing high glucose, rats in group B and group C were both under aortic balloon injury technique and fed high lipid forage post-operatively. Glucose levels and weight were observed weekly. Segments of aortoa of three groups were taken at 2, 4, 6 and 8 weeks, staining of senescent β-galactosidase (SA-β-gal) staining, HE and changes of aorta under light microscope were observed. The area of tunica intima (I) and tunica media (M) in aorta was measured, and their ratio (I/M) were analyzed. Expressions of gamma-histong family 2A variant (γ-H2AX), phosphorylated ataxia telangiectasia mutated (ATM), phosphorylated checkpoint kinasen 2 (CHK2) and phosphorylated P53 |
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ISSN: | 1672-173X |