The Role of DNA Double-strain Damage Repairing Mechanisms in Diabetic Atheroscolersis

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 48; no. 2; p. 191
• Main Authors: Zeng, Li, Ding, Qun-Fang, Xu, Ting-Yuan, Luo, Fang, Ge, Ning, Li, Shi-Tong
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2017
• Subjects: Animals; Ataxia Telangiectasia Mutated Proteins - metabolism; Atherosclerosis - genetics; Atherosclerosis - pathology; Checkpoint Kinase 2 - metabolism; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - pathology; DNA Damage; DNA Repair; Histones - metabolism; Male; Random Allocation; Rats; Rats, Wistar; Tumor Suppressor Protein p53 - metabolism; Tunica Intima; Diabetes; DNA double-strain damage repairing mechanisms; Atherosclerosis; Cellular senescence
• ISSN: 1672-173X

To identify the role of DNA double-strain damage repairing pathway in the development of diabetics atherosclerosis. Wistar male rats were randomly divided into three groups: control group (group A), balloon injury group (group B) and diabetes + balloon injury group (group C). Streptozotocin (STZ) was injected into rat abdomen to induce diabetes. After stabilizing high glucose, rats in group B and group C were both under aortic balloon injury technique and fed high lipid forage post-operatively. Glucose levels and weight were observed weekly. Segments of aortoa of three groups were taken at 2, 4, 6 and 8 weeks, staining of senescent β-galactosidase (SA-β-gal) staining, HE and changes of aorta under light microscope were observed. The area of tunica intima (I) and tunica media (M) in aorta was measured, and their ratio (I/M) were analyzed. Expressions of gamma-histong family 2A variant (γ-H2AX), phosphorylated ataxia telangiectasia mutated (ATM), phosphorylated checkpoint kinasen 2 (CHK2) and phosphorylated P53