Molecular identification in genus of Lilium based on DNA barcoding

To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experim...

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Bibliographic Details
Published inYao hsüeh hsüeh pao Vol. 49; no. 12; p. 1730
Main Authors Zheng, Si-Hao, Li, Ya-Kang, Ren, Wei-Guang, Huang, Lin-Fang
Format Journal Article
LanguageChinese
Published China 01.12.2014
Subjects
DNA Barcoding, Taxonomic
DNA, Plant - genetics
DNA, Ribosomal Spacer - genetics
Lilium - classification
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Summary:To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Lilium. ITS sequence performed higher identification efficiency, and had significant difference between intra- and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.
ISSN:0513-4870