Effect of endothelial cell-targeted soluble Notch ligand hD1R protein on the proliferation of acute myeloid leukemia cells

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 39; no. 10; pp. 845 - 850
• Main Authors: Tian, D M, Liang, Y M, Zhang, Y Q
• Format: Journal Article
• Language: Chinese
• Published: China 14.10.2018
• Subjects: Cell proliferation; Leukemia, myeloid, acute; Notch signals; Apoptosis; hD1R protein
• ISSN: 0253-2727
• DOI: 10.3760/cma.j.issn.0253-2727.2018.10.011

To evaluate the effects of endothelial cell-targeted soluble Notch ligand hD1R protein on the proliferation of acute myeloid leukemia (AML) cells. The expression levels of Notch1, Notch2, Notch3, Notch4, Hes1 in bone marrow CD34(+) cells from 24 cases of untreated AML (AML group) and 9 healthy controls (control group) were determined by real time quantitative polymerase chain reaction (PCR) . Recombinant hD1R protein was first induced and purified. Bone marrow CD34(+) cells were co-cultured on human umbilical vein endothelial cells (HUVEC) supplemented with a cocktail containing 5 types of human cytokines (5GF) and soluble hD1R. The cultured cells were tested under different culture conditions including PBS group (PBS replaces HUVEC) , hD1R group, 5GF group, GSI group (hD1R plus GSI) . Proliferation and apoptosis of cultured cells were also analyzed. Real time quantitative PCR was used to test the expression levels of Hes1 and Bcl-2 in cultured cells. The expression levels of Notch1 and Hes1 in primary AML pa