Cloning, mutagenesis and symbiotic phenotype of three lipid transfer protein encoding genes from Mesorhizobium huakuii 7653R

Lipid transfer protein superfamily is involved in lipid transport and metabolism. This study aimed to construct mutants of three lipid transfer protein encoding genes in Mesorhizobium huakuii 7653R, and to study the phenotypes and function of mutations during symbiosis with Astragalus sinicus. We us...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 56; no. 12; p. 1901
Main Authors Li, Yanan, Zeng, Xiaobo, Zhou, Xuejuan, Li, Youguo
Format Journal Article
LanguageChinese
Published China 04.12.2016
Subjects
Astragalus Plant - microbiology
Astragalus Plant - physiology
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cloning, Molecular
Gene Expression Regulation, Bacterial
Mesorhizobium - genetics
Mesorhizobium - physiology
Mutagenesis
Mutation
Nitrogen Fixation
Phylogeny
Symbiosis
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Summary:Lipid transfer protein superfamily is involved in lipid transport and metabolism. This study aimed to construct mutants of three lipid transfer protein encoding genes in Mesorhizobium huakuii 7653R, and to study the phenotypes and function of mutations during symbiosis with Astragalus sinicus. We used bioinformatics to predict structure characteristics and biological functions of lipid transfer proteins, and conducted semi-quantitative and fluorescent quantitative real-time PCR to analyze the expression levels of target genes in free-living and symbiotic conditions. Using pK19mob insertion mutagenesis to construct mutants, we carried out pot plant experiments to observe symbiotic phenotypes. MCHK-5577, MCHK-2172 and MCHK-2779 genes encoding proteins belonged to START/RHO alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC) superfamily, involved in lipid transport or metabolism, and were identical to M. loti at 95% level. Gene relative transcription level of the three genes all increased compared to free-living condition. W
ISSN:0001-6209