Apoptosis of acute myeloid leukemia HL-60 cells induced by CDK inhibitor SNS-032 and its molecular mechanisms

• Published in: Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban Vol. 44; no. 2; pp. 174 - 178
• Main Authors: Han, Yan-xia, You, Liang-shun, Liu, Hui, Mao, Li-ping, Ye, Xiu-jin, Qian, Wen-bin
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2015
• Subjects: Apoptosis; Cell Survival; Down-Regulation; Flow Cytometry; HL-60 Cells; Humans; Janus Kinase 2 - metabolism; MicroRNAs - metabolism; Oxazoles - pharmacology; Phosphorylation; Signal Transduction; STAT3 Transcription Factor - metabolism; Thiazoles - pharmacology
• ISSN: 1008-9292

To investigate the effects of cycle-dependent kinase (CDK) inhibitor SNS-032 on apoptosis in human acute myeloid leukemia (AML) HL-60 cells and its molecular mechanisms. Cultured AML HL-60 cells were treated with various concentrations of SNS-032. Cell apoptosis was determined with flow cytometry;cell viability was measured by MTT assay; the profiles of microRNA expression of HL-60 cells were analyzed by microRNA microarray;the protein expressions of JAK2/STAT3 pathway were detected by Western blotting. Apoptosis of AML HL-60 cells was induced by SNS-032; the rate of apoptosis was (5.9±1.7)%, (12.1±3.1)% and (59.4±3.6)% when HL-60 cells were treated with 0,100 and 200 nmol/L SNS-032. MicroRNA microarray analysis revealed that the levels of miR-30a, miR-183, miR-20b, miR-26b, miR-20a, miR-589, miR-107, miR-181a, miR-106a, miR-17 and miR-378c were down-regulated by SNS-032,whereas the levels of miR-320a and miR-H7* were up-regulated. Western blotting showed that SNS-032 strongly inhibited phosphorylation of STA