Effect of EPO on PRDM16, FGF21 expression and STAT phosphorylation of brown adipose tissue in HFD mice

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 34; no. 4; p. 294
• Main Authors: Zhang, Rui, Chen, Lu, Ge, Jun-Mei, Ma, Gen-Shan, Cai, Jun-Yan
• Format: Journal Article
• Language: Chinese
• Published: China 08.04.2018
• Subjects: fibroblast growth factor 21; phosphorylated signal transducer and activator of transcription 3; PR domain containing 16; brown adipose tissue; high fat diet; erythropoietin
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5654.2018.068

To investigate the effect of erythropoietin (EPO) on blood glucoseand plasma insulin level, index of insulin resistance (HOMA-IR), introperitoneal glucose tolerance test (IPGTT), the mRNA and protein level of PR domain-containing 16 (PRDM16), phosphorylated signal transducer and activator of transcription 3 (p-STAT3), fibroblast growth factor 21 (FGF21) of brown adipose tissue (BAT) in mice fed with high fat diet (HFD) in order to provide clues for the mechanism of obesity and complication. Twenty C57BL/6J male mice fed with HFD were randomly divided into control group (HFD-Con) and EPO group (HFD-EPO), mice in the two groups were injected intraperitoneally normal saline and EPO (200 IU/kg) res pectively, 3 times per week for consecutive 4 weeks.Then the body weight, blood glucose, plasma insulin level, HOMA-IR and IPGTT were detected.The mRNA and protein level of PRDM16, FGF21, p-STAT3/STAT3 in brown adipose tissue were detected by real-time quantitative RT-PCR and Western blot respectively. After intraperit