Effect of qingxin kaiqiao formula and saponin on learning and memory abilities and expression of apoptosis signal transducers Abeta and betaAPP in AD rat brain

• Published in: Zhongguo zhongyao zazhi Vol. 37; no. 19; p. 2947
• Main Authors: Chu, Sheng-hui, Hu, Hai-yan, Tan, Zhan-wang, Chen, Xiang, Wang, Wen-hua, Zhang, Xiao-yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2012
• Subjects: Alzheimer Disease - drug therapy; Alzheimer Disease - genetics; Amyloid beta-Peptides - genetics; Animals; Apoptosis - drug effects; Caspase 3 - metabolism; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; Disease Models, Animal; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Hippocampus - drug effects; Hippocampus - metabolism; Learning - drug effects; Male; Maze Learning - drug effects; Memory - drug effects; Rats; Rats, Sprague-Dawley; Saponins - administration & dosage; Saponins - pharmacology; Time Factors
• ISSN: 1001-5302

To study the effect of qingxin kaiqiao formula and saponin on the learning and memory ability and the expression of the apoptosis signal transducers Abeta and betaAPP in AD rat brain. The comparative observation method was adopted for the animal test. Forty male SD rats were randomly divided into five groups, namely the normal group, the model group, the aricept group, the qingxin kaiqiao formula group and the saponin group, with eight rats in each group. Abeta(25-35) (10 g x L(-1)) was injected into their bilateral amygdala to establish the AD rat model. Since the next day, they were intragastrically administered with Aricept (1.67 mg x kg(-1)), Qingxin Kaiqiao decoction (12.67 mL x kg(-1)), saponin (6.30 mg x kg(-1)) and double distilled water filling for 2 weeks to observe their spatial memory ability in a Morris water maze and study the expression of Caspase-3, Abeta and betaAPP in brain tissues by immunohistochemistry. Each traditional Chinese medicine groups showed significant improvement in the learnin