MicroRNA profiling in T cells of peripheral blood mononuclear cell from patients with primary biliary cirrhosis

• Published in: Zhong hua yi xue za zhi Vol. 92; no. 32; p. 2265
• Main Authors: Qian, Cheng, Wang, Huai-zhou, Fan, Hua-jie, Gu, Ming-li, Ren, Chuan-lu, Deng, An-mei, Zhong, Ren-qian
• Format: Journal Article
• Language: Chinese
• Published: China 28.08.2012
• Subjects: Adult; Case-Control Studies; Female; Gene Expression Profiling; Humans; Leukocytes, Mononuclear - metabolism; Liver Cirrhosis, Biliary - genetics; Liver Cirrhosis, Biliary - metabolism; Male; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; T-Lymphocytes - metabolism
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2012.32.010

To explore the expression pattern of microRNA (miRNA) in T cells of peripheral blood mononuclear cell (PBMC) from patients with primary biliary cirrhosis (PBC). The expression profile of miRNA in T cells of PBMC was determined by microarray assay and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In comparison with the healthy controls, 23 miRNA were down-regulated and 2 miRNA had a higher expression (all P < 0.05). As revealed by qRT-PCR, the expressions of miR-346, miR-17-5p, miR-20a and miR-let-7b decreased obviously while miR-451 and miR-129 became up-regulated. The results were in agreement with those of microarray. The PBC patients and healthy controls have significantly different expression profiles of microRNA in T cells of PBMC. The differential expression of microRNA may be involved in the pathogenesis of PBC.