CD133 promotes the invasion and metastasis of gastric cancer via epithelial-mesenchymal transition

• Published in: Zhonghua wei chang wai ke za zhi Vol. 16; no. 7; p. 662
• Main Authors: Cai, Cheng, Yu, Ji-wei, Wu, Ju-gang, Lu, Rui-qi, Ni, Xiao-chun, Wang, Shou-lian, Jiang, Bo-jian
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2013
• Subjects: AC133 Antigen; Adult; Aged; Aged, 80 and over; Antigens, CD - metabolism; Cell Line, Tumor; Epithelial-Mesenchymal Transition; Female; Glycoproteins - metabolism; Humans; Male; Middle Aged; Neoplasm Invasiveness; Neoplasm Metastasis; Peptides - metabolism; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology
• ISSN: 1671-0274

To examine the association between CD133 expression and invasion of gastric cancer, and to elucidate whether CD133 can promote the invasion and metastasis of gastric cancer via epithelial-mesenchymal transition (EMT). The CD133(+) and CD133(-) KATO-III( cells were sorted by magnetic activated cell sorting (MACS). The invasion ability was detected by Transwell method. RT-PCR and Western blot were used to detect the expression of EMT-related factors in KATO-III( cells before and after CD133 was knocked out by siRNA method. The expressions of CD133 and EMT-related proteins of cancer and adjacent normal tissues in 50 patients with gastric cancer were detected by Western blot, and correlations among protein expressions were also analyzed. As compared to CD133(-) cells, the number of broken-membrane cells was significantly higher (67.7±10.5 vs. 13.3±6.8, P=0.001) and the invasion ability was stronger (P<0.05) in CD133(+) cells, while the mRNA expression levels of Snail and N-cadherin were significantly higher in CD