Impacts on thepregnancy outcome in the mice of controlled ovarian hyperstimulation treated with acupuncture at different time points

• Published in: Zhōngguó zhēnjiǔ Vol. 36; no. 11; p. 1181
• Main Authors: Zhu, Shuxiu, Liu, Jie, Li, Changlei, Liu, Wei, Fu, Qiang, Wang, Fang, Zhang, Yuanyuan, Huang, Yingyuan
• Format: Journal Article
• Language: Chinese
• Published: China 12.11.2016
• Subjects: Acupuncture Therapy - methods; Animals; Chorionic Gonadotropin - administration & dosage; Electroacupuncture - methods; Female; Ligation; Male; Mice; Ovarian Hyperstimulation Syndrome - therapy; Ovulation Induction; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Pseudopregnancy; Random Allocation; Time Factors; Vas Deferens; acupuncture; assisted reproduction; pregnancy outcome; controlled ovarian hyperstimulation; embryo implantation
• ISSN: 0255-2930
• DOI: 10.13703/j.0255-2930.2016.11.020

To explore the optimal time of acupuncture intervention in the assisted reproduction. One hundred and twenty female mice and 60 male mice were collected. 20 female mice were selected in the natural period group and the rest 100 female mice were prepared as the model of controlled ovarian hyperstimulation (COH). The model mice were randomized into a COH group, a down-regulation group, a gonadotropins (Gn) start group, an injection of human chorionic gonadotropin (HCG) group and an embryo culture group, 20 mice in each one. The donor mice and receptor mice were subdivided in each group, 10 mice in each subgroup. One week before the experiment, vas deferens ligature was done in 30 male mice and the other 30 male mice did not receive ligature. In the down-regulation group, the Gn start group, the HCG injection group and the embryo culture group, electroacupuncture (EA) was applied to "Guanyuan" (CV 4), "Zhongji" (CV 3) and "Sanyinjiao" (SP 6) at the time points accordingly. EA stimulation was in the condition of