Effects of Sidaxue , a Miao ethnomedicine recipe, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis
To investigate the effects of (SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS). The target proteins related with RA and those involved in cell apoptosis and pyroptosis were searched in different online databases...
Saved in:
Published in | Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 41; no. 10; pp. 1473 - 1483 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
20.10.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | To investigate the effects of
(SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS).
The target proteins related with RA and those involved in cell apoptosis and pyroptosis were searched in different online databases, and Venny software was used to obtain apoptosis- and pyroptosis-related proteins in RA. RA-apoptosis-pyroptosis protein interaction (PPI) network was constructed to identity the key target proteins related with apoptosis and pyroptosis in RA. Autodock vina software was used to perform molecular docking to verify the binding ability of the main active ingredients in SX with the apoptosis- and pyroptosis-related proteins. In the cell experiment, MH7A cells were treated with 5 mg/L TGT (positive control) or 5, 10, 20, and 40 mg/L SX, and the changes in cell migration and invasion abilities and expressions of apoptosis- and pyroptosis-related proteins were examined using wound healing assay, Transwell assay, ELISA an |
---|---|
AbstractList | To investigate the effects of
(SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS).
The target proteins related with RA and those involved in cell apoptosis and pyroptosis were searched in different online databases, and Venny software was used to obtain apoptosis- and pyroptosis-related proteins in RA. RA-apoptosis-pyroptosis protein interaction (PPI) network was constructed to identity the key target proteins related with apoptosis and pyroptosis in RA. Autodock vina software was used to perform molecular docking to verify the binding ability of the main active ingredients in SX with the apoptosis- and pyroptosis-related proteins. In the cell experiment, MH7A cells were treated with 5 mg/L TGT (positive control) or 5, 10, 20, and 40 mg/L SX, and the changes in cell migration and invasion abilities and expressions of apoptosis- and pyroptosis-related proteins were examined using wound healing assay, Transwell assay, ELISA an OBJECTIVETo investigate the effects of Sidaxue (SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS). METHODSThe target proteins related with RA and those involved in cell apoptosis and pyroptosis were searched in different online databases, and Venny software was used to obtain apoptosis- and pyroptosis-related proteins in RA. RA-apoptosis-pyroptosis protein interaction (PPI) network was constructed to identity the key target proteins related with apoptosis and pyroptosis in RA. Autodock vina software was used to perform molecular docking to verify the binding ability of the main active ingredients in SX with the apoptosis- and pyroptosis-related proteins. In the cell experiment, MH7A cells were treated with 5 mg/L TGT (positive control) or 5, 10, 20, and 40 mg/L SX, and the changes in cell migration and invasion abilities and expressions of apoptosis- and pyroptosis-related proteins were examined using wound healing assay, T |
Author | Cheng, Y Liu, H Wu, N Li, M Ji, J Yuan, T Liang, X Wu, C Sun, J |
Author_xml | – sequence: 1 givenname: N surname: Wu fullname: Wu, N organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 2 givenname: T surname: Yuan fullname: Yuan, T organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 3 givenname: J surname: Ji fullname: Ji, J organization: College of Clinical Medicine, Guizhou Medical University, Guiyang 550025, China – sequence: 4 givenname: Y surname: Cheng fullname: Cheng, Y organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 5 givenname: M surname: Li fullname: Li, M organization: College of Clinical Medicine, Guizhou Medical University, Guiyang 550025, China – sequence: 6 givenname: X surname: Liang fullname: Liang, X organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 7 givenname: J surname: Sun fullname: Sun, J organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 8 givenname: H surname: Liu fullname: Liu, H organization: College of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China – sequence: 9 givenname: C surname: Wu fullname: Wu, C organization: Key Laboratory of Molecular Biology, Guizhou Medical University, Guiyang 550004, China |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34755662$$D View this record in MEDLINE/PubMed |
BookMark | eNo9kMtu1TAQhr1oRS_0FdAsWLBo0tiJ7ZMlqgpUasUCWB-Nk7HOQI4dbKfqeQcemlS9rEYz_6dfo-9MHIUYSIiPsqmlkkpd_a4551BLY9uqU7qrVaNkvcaNPhKnb-cTcZEzu0bL1jbaNO_ESdtZrY1Rp-Lfjfc0lAzRww8e8XEhuASEe8YIVHYh7mnkgQNBooFnuoQYAOc4l5g5A4YR5kOK0-FpW0t2yx4DeHYpuglzqSb-Q5APIT4wTjDQNGXgAGlHK1kij4Cp7BIXzu_Fsccp08XLPBe_vtz8vP5W3X3_env9-a6apTKl6h32etio1uoeu95r6w057zdIyttOqbFHO3bkpLPao3GtJDJ6oM1IGyupPRefnnvnFP8ulMt2z_npMwwUl7xVujfNaqszK_rhBV3camI7J95jOmxfDbb_AdLVejc |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.12122/j.issn.1673-4254.2021.10.05 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
EndPage | 1483 |
ExternalDocumentID | 34755662 |
Genre | Journal Article |
GroupedDBID | --- 2B. 2C~ 92F 92I ACGFS ALMA_UNASSIGNED_HOLDINGS CGR CUY CVF CW9 ECM EIF F5P NPM RPM TCJ TGQ U1G U5O 7X8 |
ID | FETCH-LOGICAL-p126t-9ba95c823759a49f57f6ebff8ae2f7422d9a7d4eb1b75fa6b31ee65ce8de871e3 |
ISSN | 1673-4254 |
IngestDate | Fri Apr 12 02:57:48 EDT 2024 Thu May 23 23:35:19 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Issue | 10 |
Keywords | pyroptosis apoptosis molecular docking rheumatoid arthritis Miao ehtnomedicine recipe Sidaxue data mining |
Language | Chinese |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p126t-9ba95c823759a49f57f6ebff8ae2f7422d9a7d4eb1b75fa6b31ee65ce8de871e3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 34755662 |
PQID | 2596013746 |
PQPubID | 23479 |
PageCount | 11 |
ParticipantIDs | proquest_miscellaneous_2596013746 pubmed_primary_34755662 |
PublicationCentury | 2000 |
PublicationDate | 2021-Oct-20 20211020 |
PublicationDateYYYYMMDD | 2021-10-20 |
PublicationDate_xml | – month: 10 year: 2021 text: 2021-Oct-20 day: 20 |
PublicationDecade | 2020 |
PublicationPlace | China |
PublicationPlace_xml | – name: China |
PublicationTitle | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
PublicationTitleAlternate | Nan Fang Yi Ke Da Xue Xue Bao |
PublicationYear | 2021 |
SSID | ssib051370560 ssib006562552 ssj0002923145 ssib002263520 ssib001186833 |
Score | 2.3453152 |
Snippet | To investigate the effects of
(SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis... OBJECTIVETo investigate the effects of Sidaxue (SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in... |
SourceID | proquest pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 1473 |
SubjectTerms | Apoptosis Arthritis, Rheumatoid - drug therapy Cell Proliferation Cells, Cultured Drugs, Chinese Herbal - pharmacology Fibroblasts Humans Medicine, Traditional Molecular Docking Simulation Pyrolysis Synovial Membrane |
Title | Effects of Sidaxue , a Miao ethnomedicine recipe, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis |
URI | https://www.ncbi.nlm.nih.gov/pubmed/34755662 https://search.proquest.com/docview/2596013746 |
Volume | 41 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF6FIlW9IBCv8tIilZPrEK_3ER9RKKqQ2gut1J6itb0mVsFrBRvR_gb-Dv-PmXW8cQpFwCFWZCfjtefz7Dez4xlC9sAacpHC851nqgAHRWahThQ8eFOTazWRiSgwoH90LA9P-fszcTYa_RhkLbVNOs6ufvteyf9oFfaBXvEt2X_QrBcKO-A76Be2oGHY_pWOD9bJGB_KXH9rkTDOAh0cldoGpllUtl87D7CKRe2il5h_XNu6sViLxNUJuFxaX5mka9pXgBdtU2DWTfipvDBY2MB-xeA6BvpdCu1yYeCXjS1zrJO0cLWRhkz32EVPqo_BZRmAgFwHODz8pDC2V_HbYMCFXSM_7MnWrxut80X8rNFuLBydt6u-yj4FqNxY5JotTGfFzodxDeYS69hkYIqlikOwKHxoq7siWT0mJwPLG_GuJcovUwKgkbk5AaWOvdQxnnOMiX1i-DdQcP3ZwSXmSgDXZeuJ0qcv9odukdtMJUJdCxJF2IIgHrIkYHUDVi7R6RSeVYooVpPeS0UCwZB0u_bafrTbZK-_ltd_uJIdst2P7WaPyTGnk7vkzkrN9E2H33tkdLW4T76vsEttQVfYpftUU0Qu3UAu7ZC7T21FPW4p4JZ63KIQh1t6Dbe0xy11uKVlRde4pR63D8jpu4OT2WG46gwS1hGTTZikOhEZ1lkSieZJIVQhTVoUU21YoThjeaJVzoGHpEoUWqZxZIwUmZnmZqoiEz8kW5WtzGNCpzG4RDnXUZqBZ40iVCpzJSOZTQxn0S552d_HOVheHK2ujG2_zJkA7x90x-UuedTd4HndlYiZ91p4cuORp2RnjfpnZKtZtuY58NsmfeHQ9BPft55a |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Effects+of+Sidaxue+%2C+a+Miao+ethnomedicine+recipe%2C+on+apoptosis+and+pyrolysis+of+human+fibroblast-like+synovial+cells+in+rheumatoid+arthritis&rft.jtitle=Nan+fang+yi+ke+da+xue+xue+bao+%3D+Journal+of+Southern+Medical+University&rft.au=Wu%2C+N&rft.au=Yuan%2C+T&rft.au=Ji%2C+J&rft.au=Cheng%2C+Y&rft.date=2021-10-20&rft.issn=1673-4254&rft.volume=41&rft.issue=10&rft.spage=1473&rft_id=info:doi/10.12122%2Fj.issn.1673-4254.2021.10.05&rft_id=info%3Apmid%2F34755662&rft.externalDocID=34755662 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1673-4254&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1673-4254&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1673-4254&client=summon |