Effects of Sidaxue , a Miao ethnomedicine recipe, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 41; no. 10; pp. 1473 - 1483
• Main Authors: Wu, N, Yuan, T, Ji, J, Cheng, Y, Li, M, Liang, X, Sun, J, Liu, H, Wu, C
• Format: Journal Article
• Language: Chinese
• Published: China 20.10.2021
• Subjects: Apoptosis; Arthritis, Rheumatoid - drug therapy; Cell Proliferation; Cells, Cultured; Drugs, Chinese Herbal - pharmacology; Fibroblasts; Humans; Medicine, Traditional; Molecular Docking Simulation; Pyrolysis; Synovial Membrane; pyroptosis; apoptosis; molecular docking; rheumatoid arthritis; Miao ehtnomedicine recipe Sidaxue; data mining
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2021.10.05

To investigate the effects of (SX), a recipe in Miao ethnomedicine, on apoptosis and pyrolysis of human fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS). The target proteins related with RA and those involved in cell apoptosis and pyroptosis were searched in different online databases, and Venny software was used to obtain apoptosis- and pyroptosis-related proteins in RA. RA-apoptosis-pyroptosis protein interaction (PPI) network was constructed to identity the key target proteins related with apoptosis and pyroptosis in RA. Autodock vina software was used to perform molecular docking to verify the binding ability of the main active ingredients in SX with the apoptosis- and pyroptosis-related proteins. In the cell experiment, MH7A cells were treated with 5 mg/L TGT (positive control) or 5, 10, 20, and 40 mg/L SX, and the changes in cell migration and invasion abilities and expressions of apoptosis- and pyroptosis-related proteins were examined using wound healing assay, Transwell assay, ELISA an