Pharmacokinetics and pharmacodynamics of huangqin tang in febrile rats

• Published in: Yao hsüeh hsüeh pao Vol. 49; no. 10; p. 1418
• Main Authors: Li, Tao, Wang, Yi-Wei, Wang, Yan-Li, Zhang, Dong, Zhang, Hui-Hui, Chen, Li, Zhuang, Shuai-Xing, Zhou, Zhong-Ming, Yang, Wei-Peng
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2014
• Subjects: Administration, Oral; Animals; Area Under Curve; Chromatography, Liquid; Dinoprostone - blood; Drugs, Chinese Herbal - pharmacokinetics; Fever - metabolism; Flavanones - pharmacokinetics; Flavonoids - pharmacokinetics; Glucosides - pharmacokinetics; Interleukin-1beta - blood; Mass Spectrometry; Monoterpenes - pharmacokinetics; Rats; Tumor Necrosis Factor-alpha - blood
• ISSN: 0513-4870

The pharmacodynamic (PD) and pharmacokinetic (PK) properties of Huangqin Tang (HQT) were investigated in yeast-induced febrile rats. Blood sample and rectal temperature data of the rats were collected at different times after single oral administration of HQT at 20 g x kg(-1). The plasma concentrations of paeoniflorin, baicalin, wogonoside, baicalein, wogonin, oroxylin A, glycyrrhizic acid and glycyrrhetinic acid were quantified by a sensitive liquid chromatography-tandem mass spectrometric (LC-MS) method. The blood concentrations of PGE2, 1L-1β and TNF-α were detected by radioimmunoassay (RIA). All pharmacokinetic parameters were processed by non-compartmental analysis using WinNonlin software. The potential relationship between the mean concentration of eight constituents and the antifebrile efficacy was investigated by calculating Pearson correlation coefficients. It was found that HQT had significant antifebrile efficacy in yeast-induced febrile rats, but had no effect to normal rats. The antifebrile effe