Construction of large block of engineered liver tissue seeded with co-cultured cells and in vivo implantation research

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 28; no. 3; p. 325
• Main Authors: Qu, Xiaoli, Wang, Min, He, Jiankang, Liu, Yaxiong, Zhou, Xinmin, Li, Dichen, Jin, Zhongmin
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2014
• Subjects: Animals; Cell Culture Techniques; Coculture Techniques; Collagen - chemistry; Fibroblasts - cytology; Hepatocytes - cytology; Hydrogels - chemistry; Imaging, Three-Dimensional; Implants, Experimental; Liver Cirrhosis, Experimental - therapy; Liver, Artificial; Male; Printing; Rats; Rats, Sprague-Dawley; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 1002-1892

To construct large block of engineered liver tissue by co-culture of fibroblasts and hepatocytes on collagen hydrogels in vitro and do in vivo implantation research. Silastic mould was prepared using three-dimensional printing technology. The collagen hydrogel scaffold was prepared by collagen hydrogel gel in the silicone mould and was removed. Sprague Dawley rat lung fibroblasts were co-cultured with primary hepatocytes at a ratio of 0.4 : 1 on the collagen hydrogel scaffold to construct large block of engineered liver tissue in vitro (group B), and primary hepatocytes cultured on the collagen hydrogel scaffold served as control group (group A). The cell morphology was observed, and the liver function was tested at 1, 3, 7, 14, and 21 days after culture. The rat model (n = 24) of hepatic cirrhosis was made by subcutaneous injection of carbon tetrachloride. And in vivo implantation study was carried in cirrhosis rat model. The phenotypic characteristics and functional expression of hepatocytes were evaluated