Effects of Traffic-related Air Pollution Exposure on DNA Methylation

• Published in: Huanjing kexue Vol. 38; no. 8; p. 3529
• Main Authors: Wang, Ting, Ding, Rui, Huang, Dan-Ni, Zhu, Zi-Yi, Zhang, Jun, Ye, Huai-Zhuang, Xu, Ying-Chun, Jin, Yong-Tang
• Format: Journal Article
• Language: Chinese
• Published: China 08.08.2017
• Subjects: Air Pollutants - adverse effects; Air Pollution - adverse effects; Animals; DNA Methylation; DNA Modification Methylases - metabolism; Neoplasm Proteins - metabolism; Promoter Regions, Genetic; Rats; Rats, Wistar; Seasons; Tumor Suppressor Protein p53 - metabolism; Vehicle Emissions - analysis; DNA methylation; exposure; MGMT; traffic-related air pollution; p 53
• ISSN: 0250-3301
• DOI: 10.13227/j.hjkx.201701083

The goal of the present study was to explore the effects of traffic-related air pollution exposure on DNA methylation. Into five groups of 6, 30 healthy Wistar rats were randomly divided. Three groups of rats were then exposed to traffic-related air pollution at high (tunnel), moderate (crossroad), and low (control) pollution levels for 7 d, whereas the two other groups were exposed in the tunnel for 14 d/28 d. The levels of PM and NO were measured during the exposure. The study was performed in spring and autumn, and lung tissue and blood were collected after the exposure. Promoter methylation levels of , and were quantified via pyrosequencing. The levels of PM and NO in the crossroad and tunnel groups were significantly higher than those in the control group. After 7 d exposure in autumn, promoter methylation levels of and in lung tissue significantly decreased, and the methylation status continued to decrease with increasing exposure time; was highly methylated and showed no difference among the three grou