Species identification of Echinococcus isolates collected from canines and Tibetan foxes in Chengduo County, Qinghai Province

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 31; no. 3; p. 185
• Main Authors: Feng, Kai, Huang, Fu-Qiang, Duo, Hong, Fu, Yong, Shen, Xiu-Ying, Peng, Mao, Li, Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Animals; China; DNA, Helminth - genetics; DNA, Helminth - isolation & purification; Dogs - parasitology; Echinococcus - classification; Echinococcus - genetics; Echinococcus - isolation & purification; Foxes - parasitology; Intestine, Small - parasitology; China
• ISSN: 1000-7423

To identify Echinococcus isolates collected from Tibetan foxes(Vulpes ferrilata) and dogs in Chengduo county, Yushu Prefecture, Qinghai Province. Six Tibetan foxes and 6 Tibetan dogs died accidently. Small intestines were dissected from the animals. The adult tap-worms were collected by sedimentation technique. The worms were stained with borax carmine and observed under microscope. The isolates were identified initially by morphology and the infection intensity for each animal was calculated. Eight isolates of E. multilocularis and 2 isolates of E. shiquicus were selected for the extraction of total DNA. The mitochondrion DNA CO I gene was amplified with specific primers by PCR, then sequenced and analyzed. E. multilocularis and E. shiquicus were found. Two out of 6 Tibetan foxes were infected with E. multilocularis, the infection intensity was 1,640 and 839. One Tibetan fox was infected by E. shiquicus with an infection intensity of 833. Two Tibetan dogs were infected with E. multilocularis, and the infecti