Electroacupuncture Relieves Visceral Hypersensitivity by Down-regulating Mast Cell Number，PAR-2/TRPV 1 Signaling, etc. in Colonic Tissue of Rats with Irritable Bowel Syndrome

• Published in: Zhēncì yánjiū Vol. 43; no. 8; p. 485
• Main Authors: Deng, Duo-Xi, Tan, Jie, Zhang, Hong, Huang, Gui-Lan, Li, Shuai, Guo, Kui-Kui, Wu, Yun-Yun, Yang, Bi-Ying, Xiao, Yao, Peng, Liang, Xie, Hui
• Format: Journal Article
• Language: Chinese
• Published: China 25.08.2018
• Subjects: Acupuncture Points; Animals; Cell Count; Down-Regulation; Electroacupuncture; Irritable Bowel Syndrome; Male; Mast Cells; Rats; Rats, Sprague-Dawley; Signal Transduction; Mast cells; Visceral pain; Electroacupuncture; PAR-2 /TRVP 1 signaling; Colonic tissue; Irritable bowel syndrome
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.170513

To observe the effect of electroacupuncture (EA) stimulation of "Shangjuxu" (ST 37, Lower Confluent point) and "Tianshu" (ST 25, Front-Mu point) on visceral pain and expression of colonic tryptase(Try), proteinase-activated receptor 2(PAR-2)，transient receptor potential channel vanilloid subfamily member 1 (TPRV 1)，substance P (SP) and calcitonin gene-related peptide (CGRP) in rats with irritable bowel syndrome (IBS), so as to explore its mechanisms underlying improvement of IBS. Forty male Sprague Dawley rats were equally randomized into normal control (control), model, medication and EA groups ( ＝10 in each). The IBS model was established by chronic acute combining stress (CACS, water deprivation, fasting, tail clamping, forced swimming in ice water, restraint, etc.) for 21 days. Rats of the medication group were treated by gavage of Pinaverium Bromide (1 mg/mL, 15 mg/kg), once daily for 14 d. EA (10 Hz/50 Hz, 0.2－0.3 mA) was applied to bilateral ST 37 and ST 25 for 30 min, once daily for 14 d. The muscular