Allogenic BMSCs reverse chimerism inducing the tolerance of xeno-skin graft in mouse model

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 44; no. 2; p. 165
• Main Authors: Long, Dan, Wu, Wen-Qiao, Lu, Jun, Feng, Li, Li, You-Ping, Li, Sheng-Fu
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2013
• Subjects: Animals; Bone Marrow Cells; Chimerism; Female; Immunocompromised Host; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Skin - blood supply; Skin Transplantation; Transplantation Tolerance - immunology; Transplantation, Homologous
• ISSN: 1672-173X

To investigate the feasibility of bone marrow mesenchymal stem cells (BMSCs) in inducing immune tolerance and to establish the mouse model of reverse chimerism in xeno-skin transplantation. The mouse model of bone marrow-chimerism was established with immuncompromised BALB/C-nu/nu female mice by receiving the transplantation of BMSCs from green fluorescent protein (GFP)-C57BL/10 male mice, the optimized chimeric time was identified by RT-PCR testing of SRY gene and immunohistochemistry measurement of GFP expression. In the experiment group, GFP-C57BL/10 male mice received the transplantation of the skin from immuncompromised BALB/C-nu/nu female mice with BMSCs bone marrow-chimerism. In the rejection group, GFP-C57BL/10 male mice received the transplantation of the skin from immuncompromised BALB/C-nu/nu female mice without BMSCs bone marrow-chimerism. In the control group, allo-transplantation of skin was performed in GFP-C57BL/10 male mice. Histological study was performed to investigate the survival rate an