Effect of traditional Chinese medicines with different properties on thermoregulation and temperature-sensitive transient receptor potentialion channel protein of rats with yeast-induced fever

• Published in: Zhongguo zhongyao zazhi Vol. 39; no. 19; p. 3813
• Main Authors: Wan, Hong-Ye, Kong, Xiang-Ying, Li, Xiao-Min, Zhu, Hong-Wei, Su, Xiao-Hui, Lin, Na
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2014
• Subjects: Animals; Antipyretics - administration & dosage; Antipyretics - chemistry; Body Temperature Regulation - drug effects; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - chemistry; Fever - drug therapy; Fever - immunology; Fever - microbiology; Fever - physiopathology; Gene Expression Regulation - drug effects; Humans; Male; Rats; Rats, Sprague-Dawley; Saccharomyces cerevisiae - immunology; TRPM Cation Channels - genetics; TRPM Cation Channels - immunology; TRPV Cation Channels - genetics; TRPV Cation Channels - immunology
• ISSN: 1001-5302

To compare the intervention effects of four traditional Chinese medicines (TCMs) with typical cold or hot property on body temperature and temperature-sensitive transient receptor potential ion channel proteins (TRPs) of rats with yeast-induced fever. The pyrexia model was induced by injecting yeast suspension subcutaneously. Totally 108 male SD rats were randomly divided into the normal group, the model group, the Rhei Radix et Rhizoma treated group, the Coptidis Rhizoma treated group, the Euodiae Fructus treated group, and the Alpiniae Officinarum Rhizoma treated group, with 18 rats in each group. At the 4 h, 8 h and 12 h after injection of yeast, the rats were sacrificed to collect their hypothalamus and dorsal root ganglion. The expressions of TRPV1 and TRPM8 were detected by immunohistochemistry and Western blot method. Compared with the normal group, after injection of yeast, the temperature of rats in the model group notably increased, and reached the peak at 8 h (P < 0.01). The TRPV1 level in hypothal