Internal quality control products for computer-assisted sperm analysis: Research and application

To investigate the application of the self-made semen quality control (QC) product in internal QC of computer-assisted sperm analysis (CASA). CASA was calibrated with high- and low-concentration commercially available semen QC product and meanwhile 15 samples of self-made mixed semen QC product were...

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Bibliographic Details
Published inZhonghua nan ke xue Vol. 26; no. 10; p. 895
Main Authors Ma, Ming, Zhang, Ning, Hao, Dong-Mei, Bo, Lei, Yu, Yue-Xin
Format Journal Article
LanguageChinese
Published China 01.11.2020
Subjects
Computers
Humans
Male
Quality Control
Semen Analysis - methods
Semen Analysis - standards
Sperm Motility
Spermatozoa
computer-assisted sperm analysis
sperm motion parameters
sperm concentration
semen quality control
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Summary:To investigate the application of the self-made semen quality control (QC) product in internal QC of computer-assisted sperm analysis (CASA). CASA was calibrated with high- and low-concentration commercially available semen QC product and meanwhile 15 samples of self-made mixed semen QC product were placed in 75 cryotubes containing liquid nitrogen, followed by CASA of the concentration, motility, curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN), wobble (WOB) and straightness (STR) of the sperm using standard procedures and 50 days of continuous monitoring. The Makler counting plate was used to measure the concentration and motility of the self-made sperm. The coefficients of variation (CV) of the commercially available semen QC product at high and low concentrations were 6.18% and 7.85%, respectively. CASA showed that the concentration of the self-made QC product was (25.97 ± 1.41) ×10⁶/ml, with a CV of 5.42%, and the sperm motility, VCL, VSL, VAP, LIN, W
ISSN:1009-3591