The abnormal expression of IKZF1 encoded protein-IKAROS in B-ALL children

To analyze the isoforms of IKAROS in the bone marrow samples from children with acute B-lineage lymphoblastic leukemia (B-ALL) and to investigate the relationship between frequency of dominant-negative (DN) IKAROS isoforms and prognosis of B-ALL, and to preliminarily study the relevant mechanism. A...

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Bibliographic Details
Published inZhongguo dang dai er ke za zhi Vol. 15; no. 9; p. 743
Main Authors Huang, Xiao-Hang, Chen, Jing, Li, Ben-Shang
Format Journal Article
LanguageChinese
Published China 01.09.2013
Subjects
Adolescent
Child
Child, Preschool
Female
Humans
Ikaros Transcription Factor - genetics
Infant
Male
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - metabolism
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - mortality
Prognosis
Protein Isoforms - genetics
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Summary:To analyze the isoforms of IKAROS in the bone marrow samples from children with acute B-lineage lymphoblastic leukemia (B-ALL) and to investigate the relationship between frequency of dominant-negative (DN) IKAROS isoforms and prognosis of B-ALL, and to preliminarily study the relevant mechanism. A total of 137 children with newly diagnosed B-ALL, who sequentially entered the Department of Hematology and Oncology, Shanghai Children's Medical Center between January 2005 and September 2010, were included in the study. Nest-PCR, Sanger sequencing, and TA cloning were used to analyze the expression of IKAROS isoforms in these children. The relationship between frequency of DN IKAROS isoforms and prognosis of B-ALL was investigated. Of the 137 children with newly diagnosed B-ALL, 16 had expression of IK6, 14 had expression of IK4, and 2 had expression of IK7. There was significant difference in 2.5-year event-free survival between the cohorts of DN IKAROS and non-DN IKAROS (P=0.01). Analysis of the 10 paired of di
ISSN:1008-8830
DOI:10.7499/j.issn.1008-8830.2013.09.008