Intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 33; no. 2; p. 124
• Main Authors: Xiao, Min, Qu, Xiao-Hu, Chen, Hui, Cai, Qiang-Jun, Xie, Ke-Jian
• Format: Journal Article
• Language: Chinese
• Published: China 08.02.2017
• Subjects: type 2 diabetes in mice; AdipoRon; liver; oxidative stress
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5451.2017.032

To explore the intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice, which provides basic data for clinical application. Thirty-two healthy male C57BL/6 mice were divided into 4 groups:normal group (NC, =8), diabetes mellitus group (DM, =8), high dose AdipoRon treatment group (DM + H, =8) and low dose AdipoRon treatment group (DM + L, =8). Following six weeks high fat feed, mice of DM, DM + H and DM + L were intraperitoneally injected with 40 mg/kg streptozocin (STZ), leading to type 2 diabetes. Afterwards, DM + H group and DM + L group were continuously treated with high dose and low doses of oral AdipoRon respectively for 10 days, following which, related biochemical indicators were detected. Western blot method was used to detect the p-IRS-1 protein expression in liver tissue and RT-PCR method to detect PDX-1 mRNA expression in the pancreas. The blood glucose of DM group was obviously higher than that of NC group ( < 0.05). Compared to that of DM group, blood gluco