Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 49; no. 4; p. 560
• Main Authors: Sun, Jing, Li, Can, Ma, Yan, Guo, Li-Na, Tian, Mao-Cen, Liu, Qin, Liu, Wei-Hua, Wu, Jiang, Deng, Feng-Mei, Liu, Yi-Lun
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2018
• Subjects: Animals; Liver Cirrhosis - genetics; Liver Cirrhosis - pathology; Mice; Mice, Transgenic; Zinc Finger Protein GLI1 - genetics; Hepatic fibrosis; Genetic lineage-tracing; Myofibroblasts
• ISSN: 1672-173X

To establish Gli1-CreER ; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis. Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER mice (Gli1 mice) were obtained, with Gli1-CreER ; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl . Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope. An ideal number of Gli1-CreER ; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant ( >0.05). Pseudolobular formation occurred in the CCl -induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice. Gli1-CreER ; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of