AS-PCR amplification in identification of Sedum sarmentosum with its relative species

To develop a scientific and effective method for identification of Sedum sarmentosum and its relative materials easily confused. DNA templates were extracted from plants, and DNA fragments of cpDNA trnL (tRNA-Leu) gene and trnL-trnF intergenic spacer were amplified and sequenced subsequently. trnL-t...

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Bibliographic Details
Published inZhong yao cai Vol. 37; no. 10; p. 1768
Main Authors Xie, Dong-mei, Huang, Lu-qi, Qin, Min-jian, Wang, De-qun, Jiang, Chao, Yuan, Yuan
Format Journal Article
LanguageChinese
Published China 01.10.2014
Subjects
Alleles
DNA Primers
DNA, Chloroplast - genetics
Polymerase Chain Reaction - methods
Sedum - classification
Sequence Analysis, DNA
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Summary:To develop a scientific and effective method for identification of Sedum sarmentosum and its relative materials easily confused. DNA templates were extracted from plants, and DNA fragments of cpDNA trnL (tRNA-Leu) gene and trnL-trnF intergenic spacer were amplified and sequenced subsequently. trnL-trnF sequences of all samples were aligned. The allele specific PCR method was studied with the designed allele-specific for distinguishing different samples. The result indicated that 300 bp and 500 bp DNA fragments were amplified from Sedum sarmentosum, where no any fragment was amplified from its relative species under the same reaction condition. The primers designed in this study are specific for Sedum sarmentosum from its relative species.
ISSN:1001-4454