Synthetic conformational antigen, simulating the extracellular part of M2-muscarinic receptor and its reactivity with sera from patients with idiopathic arrhythmias

• Published in: Bioorganicheskaia khimiia Vol. 39; no. 3; p. 285
• Main Authors: Pal'keeva, M E, Sidorova, M V, Molokoedov, A S, Az'muko, A A, Bespalova, Zh D, Sharf, T V, Mamochkina, E N, Efremov, E E, Rogova, M M, Mironova, N A, Zykov, K A, Golitsyn, S P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.2013
• Subjects: Amino Acid Sequence; Arrhythmias, Cardiac - blood; Arrhythmias, Cardiac - drug therapy; Arrhythmias, Cardiac - immunology; Autoantibodies - blood; Autoantibodies - immunology; Autoantibodies - isolation & purification; Humans; Peptide Fragments - chemical synthesis; Peptide Fragments - immunology; Peptide Fragments - pharmacology; Protein Conformation; Receptor, Muscarinic M2 - immunology; Receptor, Muscarinic M2 - metabolism; Receptors, Adrenergic, beta-1 - immunology; Vaccines, Synthetic - chemistry; Vaccines, Synthetic - immunology; Vaccines, Synthetic - pharmacology
• ISSN: 0132-3423
• DOI: 10.7868/S013234231303010X

Linear peptides corresponding to fragment 83-98 of the first loop and fragments 168-192 and 171-182 of the second extracellular loops of M2-muscarinic receptor (marker of early cardiac disorders and arrhythmias) were synthesized by Fmoc-SPPS method. A new conformational antigen was synthesized by method of selective ligation of linear peptides by disulfide bond with native localization. Peptides were studied in reaction with sera from patients with idiopathic arrhythmias. A new conformational antigen was recognized by sera from patients with idiopathic arrhythmias with high reactivity.