GATA-2 gene overexpression and its clinical significance in acute myeloid leukemia with AML1/ETO fusion gene

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 45; no. 4; p. 664
• Main Authors: Xie, Hui-Min, Gao, Li, Wang, Nan, Xu, Yuan-Yuan, Li, Yong-Hui, Yu, Li, Wang, Li-Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2014
• Subjects: Core Binding Factor Alpha 2 Subunit - metabolism; GATA2 Transcription Factor - metabolism; Gene Expression; Humans; Leukemia, Myeloid, Acute - diagnosis; Leukemia, Myeloid, Acute - metabolism; Oncogene Proteins, Fusion - metabolism; Polymerase Chain Reaction; Prognosis; Recurrence; RUNX1 Translocation Partner 1 Protein
• ISSN: 1672-173X

To determine the expression level of GATA-2 gene in acute myeloid leukemia with maturation (AML-M2) patients carrying AML1/ETO fusion gene. Bone marrow samples were collected from 23 patients with de novo adult AML-M2 carrying AML1/ETO fusion gene. GATA-2 gene expression was detected by real-time quantitative polymerase chain reaction (RQ-PCR). We analyzed the correlation between GATA-2 gene expression and laboratorial features and clinical prognosis of patients. GATA-2 expression levels (GATA-2 gene copies/reference gene copies) in the patients were found to be 2.17%-1 260.65%. A GATA-2 expression over or equal to 82% was defined as HIGH (10 cases), while an expression level below 82% was defined as LOW (13 cases). No significant differences were found in age, sex, white blood cell count or the rate of bone marrow blasts between HIGH and LOW expression groups (P > 0.05). Although the difference in complete remission rate between the two groups was not statistical significant (P = 0.067 8), the HIGH expressio