Berberine alleviates programmed necrosis of metabolic-associated fatty liver disease via activating Nrf2 pathway in mice

• Published in: Zhonghua gan zang bing za zhi Vol. 30; no. 2; p. 224
• Main Authors: Hao, M Y, Sun, L L, Sheng, M W, Lyu, Y S, Lin, Y B, Yang, Y H, Zhi, J H, Yu, W L, Du, H Y
• Format: Journal Article
• Language: Chinese
• Published: China 20.02.2022
• Subjects: Animals; Berberine - pharmacology; Berberine - therapeutic use; Fatty Liver; Male; Mice; Mice, Inbred C57BL; Necrosis; NF-E2-Related Factor 2 - metabolism; Metabolic associated fatty liver disease; Berberine; Necroptosis; Nuclear-factor erythroid 2-related factor 2
• ISSN: 1007-3418
• DOI: 10.3760/cma.j.cn501113-20200910-00509

To investigate the effect of berberine on programmed necrosis of hepatocytes induced by metabolic-associated fatty liver disease (MAFLD) in mice and its related molecular mechanism. Twenty male C57BL/6N mice were randomly divided into four groups (n=5 in each group): control group (S), fatty liver group (H), berberine group(B), nuclear factor erythroid 2-related factor 2 inhibitor group (Nrf2), and all-trans-retinoic acid (ATRA) group (A). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), triglycerides (TG), total cholesterol (TC), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) concentrations were detected at the end of week 12 to calculate fatty liver index (liver mass/body mass ratio). Liver tissue was stained with HE, Masson and Oil Red O, and SAF score was used to evaluate the degree of liver injury. The expression levels of hepatic programmed necrosis-related proteins, namely receptor-interacting protein kinase 3 (RIPK3), phosphorylated mixed se