Mechanism of MBL inhibiting the LPS-induced DC maturation

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 21; no. 3; p. 770
• Main Authors: Wang, Fan-Ping, Wang, Ming-Yong, Guo, Xiao-Fang, Shi, Ru-Ling, Xu, Su-Ling, Ma, Shu-Jun, Li, Hai-Bin, Guo, Ji-Qiang, Yang, Xiu-Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Cell Differentiation; Cells, Cultured; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Humans; Ligands; Lipopolysaccharides - adverse effects; Mannose-Binding Lectin - pharmacology; Monocytes - cytology; Monocytes - metabolism; Toll-Like Receptor 4 - metabolism
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2013.03.045

The study was aimed to investigate the mechanism of mannan-binding lectin (MBL) on bacterial lipopolysaccharide (LPS)-induced human peripheral blood monocyte-derived dendritic cell (DC) maturation. The monocytes were prepared from the peripheral blood of healthy adult volunteers. The immature dendritic cells (imDC) were induced by 5-day-culture in medium supplemented with rhGM-CSF and rhIL-4. FACS was used to investigate the interaction of MBL with imDC and the impact of MBL on LPS binding to imDC. ELISA and Western blot was used to analyze the interaction of MBL with soluble TLR4 ectodomain protein (sTLR4); Western blot was used to detect LPS-induced NF-κB translocation in imDC. The results showed that MBL could directly bind to imDC in the presence of calcium. sTLR4 protein or LPS could competitively inhibit the binding of MBL to imDC. ELISA and Western blot showed that MBL could evidently bind to sTLR4 protein in a concentration-dependent manner. FACS showed that MBL could competitively inhibit the binding