Effect of trichostatin A on the osteogenic differentiation potential of periodontal ligament stem cells in inflammatory microenvironment induced by tumor necrosis factor-α stimulation
To compare the expression of histone deacetylase(HDAC)1-11 of human periodontal ligament stem cells(PDLSC)in normal and inflammatory microenvironments, and to investigate the effect of histone deacetylase inhibitor trichostatin A(TSA)on the osteogenic differentiation potential of PDLSC in inflammato...
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Published in | Zhonghua kouqiang yixue zazhi Vol. 51; no. 4; p. 235 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
09.04.2016
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Abstract | To compare the expression of histone deacetylase(HDAC)1-11 of human periodontal ligament stem cells(PDLSC)in normal and inflammatory microenvironments, and to investigate the effect of histone deacetylase inhibitor trichostatin A(TSA)on the osteogenic differentiation potential of PDLSC in inflammatory microenvironment induced by tumor necrosis factor-α(TNF-α)stimulation.
PDLSC were isolated from periodontal ligament tissues obtained from the surgically extracted human teeth and cultured by single-colony selection. The expression of HDAC1-11 in cells with or without TNF-α(10 μg/L)stimulation was evaluated by quantitative real time-PCR(RT-PCR). The effect of TSA on cell proliferation was investigated by methyl thiazolyl tetrazolium(MTT)assay. The influence of TSA on osteogenic differentiation of PDLSC in inflammatory microenvironment with TNF-α stimulation was assessed by alizarin red staining, quantitative RT-PCR and Western blotting, respectively.
The expression of HDAC in PDLSC with TNF-α stimulation was sig |
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AbstractList | To compare the expression of histone deacetylase(HDAC)1-11 of human periodontal ligament stem cells(PDLSC)in normal and inflammatory microenvironments, and to investigate the effect of histone deacetylase inhibitor trichostatin A(TSA)on the osteogenic differentiation potential of PDLSC in inflammatory microenvironment induced by tumor necrosis factor-α(TNF-α)stimulation.
PDLSC were isolated from periodontal ligament tissues obtained from the surgically extracted human teeth and cultured by single-colony selection. The expression of HDAC1-11 in cells with or without TNF-α(10 μg/L)stimulation was evaluated by quantitative real time-PCR(RT-PCR). The effect of TSA on cell proliferation was investigated by methyl thiazolyl tetrazolium(MTT)assay. The influence of TSA on osteogenic differentiation of PDLSC in inflammatory microenvironment with TNF-α stimulation was assessed by alizarin red staining, quantitative RT-PCR and Western blotting, respectively.
The expression of HDAC in PDLSC with TNF-α stimulation was sig |
Author | Wang, H Jin, F Chen, Q Yang, Z H Li, D Liu, W J |
Author_xml | – sequence: 1 givenname: H surname: Wang fullname: Wang, H organization: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China – sequence: 2 givenname: Q surname: Chen fullname: Chen, Q organization: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China – sequence: 3 givenname: W J surname: Liu fullname: Liu, W J organization: State Key Laboratory of Military Stomatology, Research and Development Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China – sequence: 4 givenname: Z H surname: Yang fullname: Yang, Z H organization: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China – sequence: 5 givenname: D surname: Li fullname: Li, D organization: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China – sequence: 6 givenname: F surname: Jin fullname: Jin, F organization: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27117217$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Alkaline Phosphatase - metabolism Anthraquinones Cell Differentiation - drug effects Cell Proliferation - drug effects Core Binding Factor Alpha 1 Subunit - metabolism Gene Expression - drug effects Histone Deacetylase Inhibitors - pharmacology Histone Deacetylases - metabolism Humans Hydroxamic Acids - pharmacology Inflammation Osteogenesis - genetics Periodontal Ligament - cytology Periodontal Ligament - drug effects Staining and Labeling Stem Cell Niche Stem Cells - cytology Stem Cells - drug effects Stem Cells - enzymology Tumor Necrosis Factor-alpha - pharmacology |
Title | Effect of trichostatin A on the osteogenic differentiation potential of periodontal ligament stem cells in inflammatory microenvironment induced by tumor necrosis factor-α stimulation |
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