Effect of trichostatin A on the osteogenic differentiation potential of periodontal ligament stem cells in inflammatory microenvironment induced by tumor necrosis factor-α stimulation

• Published in: Zhonghua kouqiang yixue zazhi Vol. 51; no. 4; p. 235
• Main Authors: Wang, H, Chen, Q, Liu, W J, Yang, Z H, Li, D, Jin, F
• Format: Journal Article
• Language: Chinese
• Published: China 09.04.2016
• Subjects: Alkaline Phosphatase - metabolism; Anthraquinones; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Core Binding Factor Alpha 1 Subunit - metabolism; Gene Expression - drug effects; Histone Deacetylase Inhibitors - pharmacology; Histone Deacetylases - metabolism; Humans; Hydroxamic Acids - pharmacology; Inflammation; Osteogenesis - genetics; Periodontal Ligament - cytology; Periodontal Ligament - drug effects; Staining and Labeling; Stem Cell Niche; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - enzymology; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 1002-0098
• DOI: 10.3760/cma.j.issn.1002-0098.2016.04.010

To compare the expression of histone deacetylase(HDAC)1-11 of human periodontal ligament stem cells(PDLSC)in normal and inflammatory microenvironments, and to investigate the effect of histone deacetylase inhibitor trichostatin A(TSA)on the osteogenic differentiation potential of PDLSC in inflammatory microenvironment induced by tumor necrosis factor-α(TNF-α)stimulation. PDLSC were isolated from periodontal ligament tissues obtained from the surgically extracted human teeth and cultured by single-colony selection. The expression of HDAC1-11 in cells with or without TNF-α(10 μg/L)stimulation was evaluated by quantitative real time-PCR(RT-PCR). The effect of TSA on cell proliferation was investigated by methyl thiazolyl tetrazolium(MTT)assay. The influence of TSA on osteogenic differentiation of PDLSC in inflammatory microenvironment with TNF-α stimulation was assessed by alizarin red staining, quantitative RT-PCR and Western blotting, respectively. The expression of HDAC in PDLSC with TNF-α stimulation was sig