Determination of doxazosin enantiomers in rat plasma and investigation of their chiral inversion

The study is to establish an HPLC method using fluorescence detector for the determination of doxazosin enantiomers and investigate their chiral inversion in vitro and in vivo. Ultron ES-OVM was taken as the chiral chromatographic column, and the column temperature was 30 degrees C. Isocratic elutio...

Full description

Saved in:
Bibliographic Details
Published inYao hsüeh hsüeh pao Vol. 48; no. 6; p. 901
Main Authors Zhen, Ya-Qin, Kong, De-Zhi, Li, Qing, Zhao, Jing, Ren, Lei-Ming
Format Journal Article
LanguageChinese
Published China 01.06.2013
Subjects
Animals
Blood Chemical Analysis - methods
Doxazosin - blood
Doxazosin - chemistry
Male
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Stereoisomerism
Online AccessGet more information

Cover

More Information
Summary:The study is to establish an HPLC method using fluorescence detector for the determination of doxazosin enantiomers and investigate their chiral inversion in vitro and in vivo. Ultron ES-OVM was taken as the chiral chromatographic column, and the column temperature was 30 degrees C. Isocratic elution using a mobile phase of phosphate buffer-acetonitrile (85 : 15, v/v) at a flow rate of 0.8 mL x min(-1) was done. The fluorescence detection was set at lambda(Ex) = 255 nm and lambda(Em) = 385 nm. Prazosin was used as the internal standard. (-) Doxazosin or (+) doxazosin added into rat plasma in vitro was determined after incubating in 37 degrees C water bath for 2, 5 and 10 days. (-) Doxazosin or (+) doxazosin was administered orally to the rats for one months. Plasma samples were taken at 8 h after the last administration. A good linear relationship was achieved when the concentration of doxazosin enantiomers was within the range of 4 - 2 000 ng x mL(-1). The average recovery for (-) doxazosin was 99.5% with RS
ISSN:0513-4870