Treatment of liver cancer in vitro and in mice by monoclonal antibody targeting epithelial specific antigen-positive liver cancer stem cells in combination with cisplatin

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 38; no. 5; pp. 333 - 339
• Main Authors: He, Y Y, Yu, L, Rong, Y, Sun, L X, Sun, L C, Yang, Z H, Ran, Y L, Li, L
• Format: Journal Article
• Language: Chinese
• Published: China 23.05.2016
• Subjects: Animals; Antibodies, Monoclonal - therapeutic use; Antineoplastic Agents - therapeutic use; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - therapy; Cell Line, Tumor; Cisplatin - therapeutic use; Epithelial Cell Adhesion Molecule - antagonists & inhibitors; Epithelial Cell Adhesion Molecule - metabolism; Humans; In Situ Hybridization, Fluorescence; Liver Neoplasms - metabolism; Liver Neoplasms - therapy; Mice; Neoplastic Stem Cells - chemistry; Neoplastic Stem Cells - immunology; Organic Chemicals; Spheroids, Cellular - chemistry
• ISSN: 0253-3766
• DOI: 10.3760/cma.j.issn.0253-3766.2016.05.003

To investigate the biological characteristics of monoclonal antibodies against human liver cancer stem cells and its therapeutic effect in combination with cisplatin in the treatment of hepatocellular carcinoma. Cell culture in serum-free medium and PKH26 staining were used to determine the existence of cancer stem cells in human liver Bel7402-V3 cell line. The co-expression of antigen recognized by monoclonal antibody (McAb) 15D2 and epithelial specific antigen (ESA) and PKH26-positive cells in the Bel7402-V3 cells were detected by immunofluorescence assay. Serum-free suspension culture was used to detect the self-renewal ability of 15D2-positive Bel7402-V3 cells sorted by flow cytometry and the effect of 15D2 on the self-renewal ability of Bel7402-V3 cells. The effect of 15D2 on cisplatin resistance in the cells was examined by CCK8 method. The inhibitory effect of 15D2 combined with cisplatin on the transplanted tumor growth in mice was also observed. Single PKH26-positive cells were observed in the Bel740