Effect of alisol A 24-acetate on proliferation of aorta smooth muscle cells in rats induced by ox-LDL

• Published in: Zhongguo zhongyao zazhi Vol. 43; no. 10; p. 2147
• Main Authors: Wei, Wei, Zhou, Xiao-Mao, Wang, Yu-Cheng, Lin, Zhi-Cheng, Xue, Ji-Hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2018
• Subjects: Animals; Aorta - cytology; Cell Proliferation - drug effects; Cells, Cultured; Cholestenones - pharmacology; Lipoproteins, LDL; Muscle, Smooth, Vascular - cytology; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; Rats; cyclin; VSMC; alisol A 24-acetate; proliferation
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20180104.025

Alisol A 24-acetate, a triterpenoid extracted from Alisma orientale, has shown anti-atherosclerotic actions and many studies have proved that oxidized low density lipoprotein (Ox-LDL) could promote proliferation of aorta smooth muscle cells (VSMCs) which are closely related to atherosclerosis (AS). The purpose of this study was to evaluate the effect of alisol A 24-acetate on the proliferation of VSMCs isolated from the thoracic aorta of rats induced by ox-LDL. VSMCs were induced by ox-LDL(50 mg·L⁻¹) to establish the proliferation model and intervened by alisol A 24-acetate (5, 10, 20 mg·L⁻¹) for 12, 24 and 48 h. Then the proliferation of VSMCs was detected by MTT assay; protein expression levels of VSMCs PCNA, cyclinD1, cyclinE, p21, p27 and VSMCs PCNA, p21and p27 mRNA expression levels were detected by Western blot and Real-time polymerase chain reaction (RT-PCR) respectively. The results showed that ox-LDL could induce the proliferation of VSMCs ( <0.05), increase the protein expression levels of PCNA, cyc