Expression and significance of matriptase in ovarian cancer cells with diverse metastatic potential

• Published in: Chung-hua fu chʿan kʿo tsa chih Vol. 48; no. 5; p. 370
• Main Authors: Jiang, Zhong-qing, Chen, Xiao-fang, Sun, Peng-ming, Mao, Xiao-dan, Lin, Fen, Song, Yi-yi
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2013
• Subjects: Carcinoma, Ovarian Epithelial; Cell Line, Tumor; Cell Movement; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasms, Glandular and Epithelial - enzymology; Neoplasms, Glandular and Epithelial - genetics; Neoplasms, Glandular and Epithelial - pathology; Ovarian Neoplasms - enzymology; Ovarian Neoplasms - genetics; Ovarian Neoplasms - pathology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Serine Endopeptidases - genetics; Serine Endopeptidases - metabolism
• ISSN: 0529-567X

To study the expression and significance of matriptase in different metastatic potential of human ovarian cancer cells. High-metastatic human ovarian cancer cell HO8910PM and ovarian cancer cell HO8910 were collected.The ability of metastatic of the former was stronger than that of the latter. Compared the ability of invasion and migration in HO8910PM and HO8910 by scratch assay and by millicell chamber artificial reconstituted basement membrane invasion assay. Detected the matriptase mRNA and protein expression levels in HO8910PM and HO8910 through reverse transcription(RT)-PCR and immunocytochemistry methods. The 24 hours' migration distance (347 ± 8) µm of HO8910PM cells were significantly higher than that in HO8910 group (154 ± 10) µm (P < 0.01);The number of HO8910PM cells that penetrated the matrigel after 24 hours' incubation were significantly higher than that in HO8910 group (90.7 ± 2.1 vs 63.3 ± 1.5,P < 0.01). The expression of matriptase mRNA in HO8910PM cells was higher than that in HO8910 group (