Molecular of pulmonary arterioles relaxation through SUR2B/Kir6.1 channel opening

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 32; no. 3; p. 238
• Main Authors: Chen, Jun, Wang, Shang, Cui, Wen-Yu, Long, Chao-Liang, Zhang, Yan-Fang, Zhang, Hao, Wang, Hai
• Format: Journal Article
• Language: Chinese
• Published: China 08.03.2016
• Subjects: Animals; Arterioles - drug effects; Arterioles - physiology; Dilatation; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Epoprostenol; Glyburide - pharmacology; Indomethacin - pharmacology; KATP Channels - physiology; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide; Rats; Sulfonylurea Receptors - physiology; pulmonary arterioles; endothelial cells; rats; Iptakalim; ATP-sensitive potassium channel
• ISSN: 1000-6834
• DOI: 10.13459/j.cnki.cjap.2016.03.013

To study the dilatation characteristics of ATP-sensitive potassium channel (K ) SUR2B/Kir6.1 subtype opener iptakalim (Ipt) in pulmonary arterioles, and to explore its possible mechanism. Vessels pressure-diameter monitoring perfusion technique was used to observe the dilatation effects of Ipt in rats fourth pulmonary arterioles ( =6~8). After the pulmonary arterioles were pre-treated with removing endothelium or pre-incubated with K channel blocker glibenclamide (Gli), cyclo-oxygenase (COX) inhibitor indomethacin (Indo) and nitric oxide synthase (NOS) inhibitor L-Nω-Nitro-arginine methyl ester(L-NAME), the dilatation effects of Ipt were observed. Pulmonary arterioles could be relaxed by Ipt, the maximal relaxation rate was (60.53±2.08)%. Compaired with control group, the effects of Ipt in endothelium denuded arterioles were significantly decreased, the maximal relaxation rate was (9.47±1.56)% ( <0.01). The maximal relaxation rate were decreased to(17.49±1.47)%,(37.00±3.88)% and(24.91±2.30)% respectively afte