Effect of electroacupuncture and moxibustion pretreatment on expression of cerebral micro- RNAs and Aquaporin protein-4 in cerebral infarction rats

• Published in: Zhēncì yánjiū Vol. 40; no. 2; p. 99
• Main Authors: Zheng, Shi-Ping, Han, Wei, Chu, Hao-Ran, Wang, Ying, Zhang, Ling, Zhu, Ling-Ling, Zhang, Guo-Qing, Cui, Qian-Qian
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2015
• Subjects: Acupuncture Points; Animals; Aquaporin 4 - genetics; Aquaporin 4 - metabolism; Brain Ischemia - genetics; Brain Ischemia - metabolism; Brain Ischemia - prevention & control; Brain Ischemia - therapy; Combined Modality Therapy; Electroacupuncture; Humans; Male; MicroRNAs - genetics; MicroRNAs - metabolism; Moxibustion; Rats; Rats, Wistar; Up-Regulation
• ISSN: 1000-0607

The present study aimed at observing the effect of moxibustion pretreatment on the expression of cerebral microRNAs and Aquaporin protein-4 (AQP 4) in rats with cerebral ischemia and reperfusion (CI/R), so as to reveal its mechanism underlying improvement of cerebral infarction. A total of 130 Wistar rats were randomly divided into blank control (n = 10), CI/R model (n = 30), electroacupuncture (EA, n = 30), moxibustion (n = 30), Aspirin groups (n = 30). Before modeling, EA (2 Hz/5 Hz, 1-2 mA) or moxibustion was applied to "Baihui" (GV 20), "Fengfu" (GV 16) and "Dazhui" (GV 14) for 20 min, once daily for 7 days. The rats of the Asprin group were treated by intragastric administration of Aspirin (10 mg/kg, 1 mg/mL) , once daily for 7 days before modeling. The CI/R model was established by occlusion of the bilateral carotid arteries. The expression levels of cerebral miRNAs and AQP 4 were detected by real-time PCR and Western blot, respectively. Compared with the blank control group, the expression levels of ce