Cloning and Bioinformatics Analysis of Toxoplasma gondii ROP21 Gene

The full-length gene sequence of Toxoplasma gondii ROP21 (TgROP21) gene was amplified with PCR. The signaling peptide and transmembrane domain of TgROP21 protein were predicted by SignaIP and TMHMM online predictive sites, and the hydrophilicity and antigenic index of this protein were ananlyzed wit...

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Bibliographic Details
Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 33; no. 4; p. 294
Main Authors Shi, Shi-jun, Cui, Yong, Li, Jin, Wang, Hong-fa, Yin, Kun, Wei, Qing-kuan, Huang, Bing-cheng, Sun, Hui, Liu, Gong-zhen
Format Journal Article
LanguageChinese
Published China 01.08.2015
Subjects
Cloning, Molecular
Computational Biology
Genes, Protozoan
Polymerase Chain Reaction
Toxoplasma
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Summary:The full-length gene sequence of Toxoplasma gondii ROP21 (TgROP21) gene was amplified with PCR. The signaling peptide and transmembrane domain of TgROP21 protein were predicted by SignaIP and TMHMM online predictive sites, and the hydrophilicity and antigenic index of this protein were ananlyzed with DNAStar software. Meanwhile, the functional domains and tertiary structure were modeled by combined use of ExPASY and PRODATA online sites. As expected, the PCR results revealed one band at 2,022 bp. The signaling peptide, transmembrane domain, hydrophilicity, antigen index, functional domain and 3D structure of TgROP21 were successfully predicted. This work may provide a theoretical foundation for further verification of TgROP21 function.
ISSN:1000-7423