Pro-apoptotic effects of luteolin on hepatoma HepG2 cells

• Published in: Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban Vol. 42; no. 5; p. 504
• Main Authors: Wang, Yuan-Peng, Zhou, Liang, Gong, Xing-Guo
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2013
• Subjects: Apoptosis - drug effects; Hep G2 Cells; Humans; Luteolin - administration & dosage; Luteolin - pharmacology; Reactive Oxygen Species - metabolism
• ISSN: 1008-9292

To investigate the effect of luteolin on cell growth and apoptosis of HepG2 cells in vitro. Cultured HepG2,HL60,A549 and LO2 cells were treated with luteolin for different doses (0 μg/ml,2.5 μg/ml,10 μg/ml and 20 μg/ml) and varied times (0 h,24 h,48 h and 72 h). Cell viability was measured with MTT assay and IC50 was calculated. The reactive oxygen species (ROS) levels in HepG2 cells treated with luteolin for 6 h and 12 h were measured with flow cytometry. Cell apoptosis of HepG2 cells treated with luteolin for 24h was examined with flow cytometry and Annexin V-FITC/PI. Expression levels of apoptosis pathway proteins (p53,ASPP2 and iASPP) in HepG2 cells were detected with western blot and the dose and time-effect was analyzed. Luteolin effectively inhibited tumor cell proliferation in a dose-and time-dependent manner,and the inhibition rates of 20 μg/ml Luteolin for 72 h were 39.34%,62.90%,57.57% and 62.90% to LO2,HepG2, HL60 and A549 cells,respectively. The intracellular ROS level was decreased in HepG2 cell