Optimization of experimental parameters for quantitative NMR (qNMR) and its application in quantitative analysis of traditional Chinese medicines

Quantitative NMR (qNMR) is a technology based on the principle of NMR. This technology does not need the references of the determined components, which supplies a solution for the problem of reference scarcity in the quantitative analysis of traditional Chinese medicines. Moreover, this technology h...

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Bibliographic Details
Published inYao hsüeh hsüeh pao Vol. 49; no. 9; p. 1248
Main Authors Ma, Xiao-Li, Zou, Ping-Ping, Lei, Wei, Tu, Peng-Fei, Jiang, Yong
Format Journal Article
LanguageChinese
Published China 01.09.2014
Subjects
Biological Products - chemistry
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal - chemistry
Magnetic Resonance Spectroscopy - methods
Medicine, Chinese Traditional
Reproducibility of Results
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Summary:Quantitative NMR (qNMR) is a technology based on the principle of NMR. This technology does not need the references of the determined components, which supplies a solution for the problem of reference scarcity in the quantitative analysis of traditional Chinese medicines. Moreover, this technology has the advantages of easy operation, non-destructiveness for the determined sample, high accuracy and repeatability, in comparison with HPLC, LC-MS and GC-MS. NMR technology has achieved quantum leap in sensitivity and accuracy with the development of NMR hardware. In addition, the choice of appropriate experimental parameters of the pre-treatment and measurement procedure as well as the post-acquisition processing is also important for obtaining high-quality and reproducible NMR spectra. This review summarizes the principle of qHNMR, the various experimental parameters affecting the accuracy and the precision of qHNMR, such as signal to noise ratio, relaxation delay, pulse width, acquisition time, window function,
ISSN:0513-4870