Effect of HIF-2α on the biological characteristics of breast cancer stem cells

• Published in: Zhong hua yi xue za zhi Vol. 98; no. 4; p. 269
• Main Authors: Liu, Y Q, Li, N, Huang, H F, Li, H X
• Format: Journal Article
• Language: Chinese
• Published: China 23.01.2018
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors; Breast; Breast Neoplasms; Cell Line, Tumor; Humans; MCF-7 Cells; Mice; Mice, Nude; Neoplastic Stem Cells; Breast cancer; HIF-2α; MCF-7; Stem cell
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2018.04.006

To explore the effect of hypoxia inducible factor (HIF)-2α on biological characteristics of breast cancer stem cells (BCSCs). Stem cells were isolated and purified from MCF-7 cells by using the immunomagnetic microbeads. HIF-2α ORF/shRNA lentiviral vectors were transduced into MCF-7 stem cells respectively, and then the stable stem cell lines were detected and gained. Using the method of Cell Counting Kit-8 (CCK-8) and flow cytometry, the effect of HIF-2α on cell vitality and apoptosis of MCF-7 cancer stem cells (CSCs) were tested. Serum-free suspension culture was used on MCF-7 cells to get breast CSCs microspheres. The expression of CD44(+) in different groups were detected by flow cytometry. Tumor-bearing nude mice model was established to compare tumor growth rate and pulmonary metastasis probability of MCF-7 CSC HIF-2α knock up/down . Compared with MCF-7 CSC group, the apoptosis rate of MCF-7 CSCs HIF-2α knockup group decreased obviously and cell proliferation activity increased significantly (all <0.05)