Clinical significance of MP-DNA from endotracheal aspirates in diagnosis of Mycoplasma pneumoniae pneumonia in children

• Published in: Zhongguo dang dai er ke za zhi Vol. 17; no. 9; p. 937
• Main Authors: Gu, Wen-Jing, Zhang, Xin-Xing, Chen, Zheng-Rong, Yan, Yong-Dong, Zhu, Can-Hong, Huang, Li, Wang, Mei-Juan, Shao, Xue-Jun, Ji, Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2015
• Subjects: Adolescent; Bronchoalveolar Lavage Fluid - microbiology; Child; Child, Preschool; DNA, Bacterial - analysis; Female; Humans; Infant; Male; Nasopharynx - microbiology; Pneumonia, Mycoplasma - diagnosis
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2015.09.010

To compare the detection rates of Mycoplasma pneumoniae (MP) from nasopharyngeal aspirates (NPA) and bronchoalveolar lavage fluid (BALF) in children with pneumonia. A total of 164 hospitalized children with pneumonia were enrolled. NPA and BALF of these children were collected within 24 hours of admission, and MP-DNA was detected by fluorescence quantitative PCR. Venous blood samples of all these children were collected within 24 hours of admission and on days 7-10 of treatment, and serum MP-IgM was detected using ELISA. The positive rate of MP-DNA in NAP of the 164 cases was 51.8% , which was lower than 63.4% as the detection rate of MP-IgM in serum (P=0.044), and the two detection rates were moderately consistent with each other (Kappa=0.618, P<0.01). The positive rate of MP in BALF was 71.3%, which was not significantly different with that of MP-IgM in serum (P>0.05), and the detection rates were well consistent (Kappa=0.793, P<0.01). The detection rate of MP in NPA was lower than that in BALF (P<0.01), wi