The effect of ph on the fertilizability of rainbow trout (Oncorhynchus mykiss) eggs stored in a chilled state

To verify the observation that fertilizability of stored rainbow trout eggs is affected by deviations in pH (Komrakova and Holtz, 2011) freshly collected eggs were immersed in coelomic fluid adjusted to pH 6, 7, 8, 9, or 10 (3 replicates each) and stored at 2 degreesC. At 0, 1, 24, 48, and 96h, pH w...

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Bibliographic Details
Published inCommunications in agricultural and applied biological sciences Vol. 78; no. 4; p. 215
Main Authors Komrakova, M, Kuembet, U, Holtz, W
Format Journal Article
LanguageEnglish
Published Belgium 2013
Subjects
Animals
Fertilization
Fisheries - methods
Hydrogen-Ion Concentration
Oncorhynchus mykiss - physiology
Ovum - physiology
Refrigeration
Water - chemistry
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Summary:To verify the observation that fertilizability of stored rainbow trout eggs is affected by deviations in pH (Komrakova and Holtz, 2011) freshly collected eggs were immersed in coelomic fluid adjusted to pH 6, 7, 8, 9, or 10 (3 replicates each) and stored at 2 degreesC. At 0, 1, 24, 48, and 96h, pH was measured and eggs were inseminated with cryopreserved semen. Egg development until the eyed stage proceeded normally in all cases. However hatching rates, amounting to 70% in controls (pH 8, SEM 0.02), were reduced to 50% in samples exposed to pH 7 or pH 9 and to 40% and 30% in samples exposed to pH 6 and pH 10, respectively. In conclusion, when eggs are subjected to coelomic fluid with deviating pH, the damage afflicted upon the eggs will not become evident until they are due to hatch.
ISSN:1379-1176