Modulatory Effect of Mouse Compact Bone-derived Suspending MSC on T Cells and It's Related Mechanisms

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 24; no. 2; p. 584
• Main Authors: Li, Xin, Wu, Wen-Qing, Ding, Li, Liu, Yuan-Lin, Mao, Ning, Zhang, Yi, Zhu, Heng, Ning, Shou-Bin
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2016
• Subjects: Adipocytes - cytology; Animals; Cell Adhesion; Cell Culture Techniques; Cell Differentiation; Cell Proliferation; Culture Media, Conditioned; Flow Cytometry; Immunophenotyping; Interleukin-6 - metabolism; Lymphocyte Activation; Mesenchymal Stromal Cells - cytology; Mice; Osteoblasts - cytology; T-Lymphocytes - cytology; T-Lymphocytes - metabolism; Transforming Growth Factor beta1 - metabolism
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2016.02.052

To investigate the modulatory effect of the MSC derived from low attaching culture systems (suspending MSC) on T lymphocytes and the related mechanisms. The suspending MSC were generated from mouse compact bones by using low attaching plates and adherent cell culture flasks, respectively. The morphology of suspending MSC was observed under the inverted microscope and the cells were induced to differentiate into osteoblasts and adipocytes. Further, the surface antigen profile of MSC was analyzed with flow cytometry. In addition, the culture medium (CM) of suspending MSC and adherent MSC was collected and added into the activated T cell cultures before detection of the proliferation by CFSE assay. Moreover, the modulaory effects of the CM on the T cell-derived cytokines were detected by quantitative PCR. Also, the mRNA expression of cytokines of MSC was detected. The suspending MSC grew in floating cell spheres and differentiated into osteoblasts and adipocytes in the induction medium. Furthermore, the suspendi