Intervention of Tibetan medicine--twenty wei chenxiang pill on elevation of hypoxic pulmonary arterial pressure in rats

• Published in: Zhong yao cai Vol. 37; no. 9; p. 1640
• Main Authors: Jin, Guo-en, Yang, Quan-yu, Cao, Cheng-zhu, Yan, Yun-fei, Yang, Chun-yan, Li, Sheng-hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2014
• Subjects: Animals; Arterial Pressure; Hypertension, Pulmonary; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit; Lung; Medicine, Tibetan Traditional; Pulmonary Artery; Rats; Rats, Wistar
• ISSN: 1001-4454

To investigate the role of Tibetan medicine-Twenty Wei Chenxiang Pill interfering with serum ET-1 level, in order to confirm that ET-1 is involved to the pathogenesis of hypoxic pulmonary hypertension. 165 Wistar rats were randomly divided into high altitude control group,Tibetan medicine-Twenty Wei Chenxiang Pill group and plain control group. The physiological signal acquisition system was used to record pulmonary arterial pressure, and RV/(LV + S) ratio were caculated. Serum HIF-1alpha and ET-1 protein levels were determined by the method of ELISA, and ETA protein levels in lung tissue were determined by Western Blot method. Compared with the high altitude group,in the rats of Tibetan medicine-Twenty Wei Chenxiang Pill group,the pulmonary arterial pressure decreased significantly from the seventh day and the seventh day (P < 0.01), the RV/(LV + S) ratio and serum HIF-1alpha levels decreased significantly from the third day (P < 0.05 or P < 0.01), the serum ET-1 levels decreased significantly from the third