Comparison of the characteristics of NK cells after two different methods of expansion and observation of the clinical efficacy in patients who relapsed post allogeneic hematopoietic stem cell transplantation

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 43; no. 5; p. 400
• Main Authors: Cao, X H, Wang, Z D, Sun, Y Q, Kong, J, Lu, S Y, Tang, F F, Zhang, Y Y, Wang, J Z, Xu, L P, Zhang, X H, Wang, Y, Liu, K Y, Huang, X J, Zhao, X Y
• Format: Journal Article
• Language: Chinese
• Published: China 14.05.2022
• Subjects: Antibodies, Monoclonal - metabolism; Antibodies, Monoclonal - pharmacology; Graft vs Host Disease - metabolism; Hematopoietic Stem Cell Transplantation; Humans; Killer Cells, Natural; Retrospective Studies; Treatment Outcome; NK cells; Relapse; infusion; Leukemia; expansion
• ISSN: 0253-2727
• DOI: 10.3760/cma.j.issn.0253-2727.2022.05.009

To explore the differences in the biological effects of different expansion systems on natural killer (NK) cells, as well as the safety and preliminary clinical efficacy in the treatment of patients with recurrence after allogeneic hematopoietic stem cell transplantation (allo-HSCT) . Peripheral blood cells from healthy donors were stimulated with either CD3 combined with CD52 or K562 feeder cells loaded with IL-21/4-1BB to induce NK cell expansion. Changes in the NK cell phenotype, cytokine secretion, and cytotoxicity before and after expansion were detected. We also evaluated the safety and clinical efficacy of two different expansion strategies for patients received NK infusion. Compared with the CD3/CD52 monoclonal antibody amplification system, the feeder cell expansion group had a higher purity of NK cells and higher expression ratios of NK cell surface activation receptors such as DNAM-1 and NKp30, while inhibitory receptor CTLA-4 expression was low and NKG2D/CD25/CD69/ Trail/PD-1/TIM-3/TIGIT had no st