Analysis of the protein expression and gene amplification of HER2 in gastric cancer

To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer. Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients...

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Published inZhonghua wei chang wai ke za zhi Vol. 16; no. 9; p. 898
Main Authors Liu, Jun-lin, Li, Zhen-kai, Xin, Fang-jie, Zhao, Han, Zhao, Wei, Gao, Yuan, Hu, Ji-lin, Zhang, Xian-xiang, Zhang, Mao-shen, Zhang, Bing-yuan, Lu, Yun
Format Journal Article
LanguageChinese
Published China 01.09.2013
Subjects
Adult
Aged
Female
Gene Amplification
Humans
Male
Middle Aged
Receptor, ErbB-2 - genetics
Receptor, ErbB-2 - metabolism
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
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Summary:To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer. Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients. Protein expression of HER2 was negative in 51 cases, (+) in 12 cases, (++) in 12 cases, (+++) in 5 cases, and the positive expression rate was 21.3% (17/80). Seven (8.8%) cases had gene amplification of HER2, including gene critical amplification in 3 (3.8%) cases. The result of IHC was positively correlated with CISH (P<0.05), and the coincidence rate was 85.0% (68/80). HER2 positive expression rate was higher in the gastroesophageal junction carcinoma, poorly differentiated and stage III-IIII gastric cancer (all P<0.05). The gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2, which is closely correlated to the development of gastric cancer.
ISSN:1671-0274