Effect of Human Adipose Mesenchymal Stem Cells on Phenotype Polarization of Mice Microglia via TLR3/TRIF Signal Pathway

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 50; no. 2; p. 164
• Main Authors: Sun, Jing, Liu, Yi-Lun, Li, Can, Ma, Yan, Yan, Wei-Heng, Su, Bing-Yin
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2019
• Subjects: Adaptor Proteins, Vesicular Transport - metabolism; Adipose Tissue - cytology; Animals; Cell Polarity; Cells, Cultured; Humans; Lipopolysaccharides; Mesenchymal Stem Cells - cytology; Mice; Mice, Inbred C57BL; Microglia - cytology; Phenotype; Signal Transduction; Toll-Like Receptor 3 - metabolism; Human adipose derived mesenchymal stem cells (hADMSCs); Phenotype; Toll-like receptors (TLRs)
• ISSN: 1672-173X

To explore the effect and mechanism of human adipose-derived mesenchymal stem cells (hADMSCs) on phenotypic polarization of microglia. BV-2 microglia of C57/BL6 mice were co-cultured with hADMSCs+lipopolysaccharide (LPS), or cultured with LPS alone. Cell morphology was observed under an inverted microscope. The effect of hADMSCs on microglial proliferation was evaluated by CCK-8 assay. The impact of hADMSCs on microglia M1/M2 phenotype markers were detected using quantitative real-time PCR (RT-qPCR). The affect of hADMSCs on the proteins expression levels of Toll-like receptor 4 (TLR4)-TIR domain containing adaptor protein inducing interferon β (TRIF) signaling pathway in BV-2 microglia was detected by using Western blot analysis. As compared with the LPS treatment, hADMSCs treatment had no obvious effect on microglia morphology, whereas showed significant inhibition on microglial proliferation activity ( <0.05). Simultaneously, hADMSCs treatment reduced expression of microglia M1 phenotype markers ( <0.05),