Expression rhythm of autophagic gene in neurons of neonatal rats with hypoxia/ischemia and its regulatory mechanism

• Published in: Zhongguo dang dai er ke za zhi Vol. 19; no. 8; pp. 938 - 944
• Main Authors: Li, Shi-Ping, Zhu, Jiang-Hu, Zhao, Feng-Yan, Zheng, Zhen, Mu, De-Zhi, Qu, Yi
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2017
• Subjects: Animals; Animals, Newborn; Autophagy - genetics; Beclin-1 - genetics; Circadian Rhythm; Female; Hypoxia-Ischemia, Brain - metabolism; Male; Microtubule-Associated Proteins - genetics; Neurons - metabolism; Rats; Rats, Sprague-Dawley
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2017.08.017

To investigate the expression of autophagic gene and circadian gene in the neurons of neonatal rats after hypoxic-ischemic brain damage and the mechanism of nerve injury induced by hypoxia/ischemia. Twelve Sprague-Dawley (SD) rats were randomly divided into hypoxic-ischemic (HI) group and sham-operation group, with 6 rats in each group. Ligation of the right common carotid artery and hypoxic treatment were performed to establish a model of hypoxic-ischemic brain damage. Western blot was used to measure the expression of the circadian protein Clock in the cortex and hippocampus. The neurons of the rats were cultured in vitro and randomly divided into oxygen glucose deprivation (OGD) group and control group. The neurons in the OGD group were treated with DMEM medium without glucose or serum to simulate ischemic state, and hypoxic treatment was performed to establish an in vitro model of hypoxic-ischemic brain damage. Western blot was used to measure the expression of autophagy-related proteins Beclin1 and LC3 a